Creatine Kinase Activity in Human Seminal Fluid
نویسندگان
چکیده
Introduction: Male infertility is an important factor of infertility in couples. In most cases it is caused by defects in sperm cell function; whether low sperm cell count or reduced motility along with altered morphology. Defects in sperm cells development are influencing enzyme activities in the seminal fluid. Aim: This research tried to determine can activity of creatine kinase (CK) in seminal fluid be used as a marker of fertilizing ability in infertile the region of the plane Dukagjini in Kosovo, and to determine the link between that activity and the quality of sperm cells.Materials and methods: Seminal fluid and blood samples were taken from 200 patients, 150 of whom were infertile with confirmed oligozoospermia, oligoasthenozoospermia and azoospermia. Sperm cells number, motility and morphology, CK activity in seminal fluid and concentrations of gondaotropic hormones were analysed in comparison to values obtained from 50 normozoospermic subjects used as a control. Results: CK activity in seminal fluid of patients with primary and secondary infertility is significantly higher than that of the control group (p<0,005). CK activity depended on sperm cells number, motility and morphology. Higher ratio of abnormal sperm cells parameters correlated with higher CK activity in seminal fluid. Conlusion: Enzymatic activity of CK in seminal fluid is a valuable biochemical marker in determining fertilizing ability of sperm cells. This biochemical marker is used in clinical assesment of sperm cells fertilizing potential and represents an important diagnostic feature.
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